Improving Mobility and Function Following Transfemoral Amputation: A Novel Approach to Reverse Volumetric Muscle Loss

Inclusion Criteria: * At least 18 years old * History of lower limb unilateral transfemoral amputation. * At least two years post lower limb amputation * Able to ambulate independently without the use of aids (i.e., walking cane). * Able to walk at least 10 minutes continuously without stopping. * Has a safe residuum with no open wounds on either the residual or intact limb. * Has a prosthetic limb with materials that are sound and safe to withstand the mobility requirements of the study. Exclusion Criteria: * Younger than 18 years old. * Inability to give informed consent. * Neurological disorder that affects gait. * Participants with significant metal (greater than one orthopaedic screw) in either the residual limb or the sound/intact limb * Participants who begin taking medications or change dosage of medications that could affect gait, balance, and cardiovascular function during the course of the study. * Currently pregnant (or intend to become pregnant while participating in study). * History of any condition where fatiguing contractions or resisted leg contractions are contraindicated. * Blood clots in the leg, or any condition in which compression of the thigh or transient ischemia is contraindicated (e.g. open wounds in the leg). * History of uncontrolled hypertension. * History of heart failure. * Head injury within the previous 6 months. * Seizure disorder. * History of vascular disease. * History of thrombosis. * History of sickle cell trait. * History of genetic disease