Predicting Treatment Outcomes in Refractory Constipation Through Brain Connectivity Evaluation

Inclusion Criteria: * 18≤ age ≤ 45 years old * Right-handed * Patients diagnosed as functional constipation according to the Rome IV criteria * Informed consent of patients Exclusion Criteria: * Complicated with gastrointestinal organic disease or significant functional abnormalities (tuberculosis, polyps, Crohn's disease, tumors, congenital megacolon, pelvic floor muscle relaxation, abnormal colonic transit test, etc.) * Long-term intense exercise (continuous exercise for more than 8 hours per week, such as marathon runners or triathletes) * No history of chronic pain, no recent major trauma * Drug abuse or tobacco dependence (half a pack or more per day) * Combined hypothyroidism and Parkinson's disease * Patients with confirmed mental illness or neurological disorders who take psychotropic drugs, analgesics or hormones * History of abdominal surgery (appendectomy, hysterectomy, or cholecystectomy) * Contraindications to functional magnetic resonance imaging (claustrophobia, metal implants) * Pregnant or lactating women with constipation after delivery * Patients with other benign and malignant tumors and autoimmune diseases * Infectious diseases such as hepatitis B, hepatitis C, AIDS, etc. * Heart disease, organ failure and other chronic diseases that require long-term medication or affect the quality of life