Effect of Force Magnitude on Canine Retraction and Gingival Crevicular Fluid Proteome

Inclusion Criteria: * Healthy patient, non-smoker * Age: 11 - 16 years of age, inclusive. * A full complement of dentition (central incisor to 1st molar) in the four quadrants, except second molars * Patients requiring at least upper bilaterally maxillary 1st premolar extraction as a treatment plan (Class I bimaxillary protrusion/ crowding or Class II maxillary dentoalveolar protrusion/ crowding) * At least 5 mm of space left between canine and 2nd premolar to be closed at the time of canine retraction * At least six months after the extraction of first premolars and in a 0.019 x 0.025-inch stainless steel archwire in the maxilla * The ability to read and understand English and to provide informed consent Exclusion Criteria: * Patients under 11 years of age and over the age of 16. * Non-extraction treatment plan * Extraction of maxillary tooth other than first premolars bilaterally. * Evidence of poor oral hygiene * Evidence of previous root resorption * Missing or impacted tooth in any of the quadrant except 3rd molars. * Medical issues that affect tooth movement * Inability to provide oral and written consent to participate. * Patient on medications that may alter bone metabolisms like bisphosphonates, oral contraceptives, or PTH (parathyroid hormone) * Chronic illness or syndromic patients. * Pregnant women, prisoners, and decisional impaired