Kombucha in Overweight and Obese: Live Vs. Pasteurized Effects on Microbiota, Metabolism, and Liver Function

Inclusion Criteria: Individuals with a Body Mass Index (BMI) between 25 kg/m² and 34.9 kg/m², of both biological sexes, aged between 18 and 60 years, available to comply with the study protocol (described in this document) and sign informed consent. Exclusion Criteria: Volunteers will be excluded from the study if they present one or more of the following conditions: 1. Subjects with sensitivity to kombucha; 2. Consumption of kombucha, kefir, kimchi, cheese, raw vinegar, sauerkraut, kvass, and other fermented products during the study and in the 3 weeks before the study. 3. Use of antibiotics in the 6 months prior to the start of the study; 4. Use of pro/prebiotics or fibers as dietary supplements or any food/molecule that modifies intestinal transit time 6 weeks before recruitment; use of laxatives 6 weeks before recruitment; 5. Specific dietary regimen (e.g., vegan); specific dietary treatment (e.g., high protein); 6. Excessive consumption of substances and alcohol; smokers; 7. Diagnosis of gastrointestinal disorders, hormonal or thyroid diseases, autoimmune diseases, and/or chronic use of corticosteroids; psychiatric disease; Type 1 or 2 diabetes; 8. Use of proton pump inhibitors; antidiabetic drugs or insulin and statins; 9. Subjects with insulin sensitivity; 10. Pregnant or lactating women; 11. Subjects with tooth sensitivity 12. Participation in another clinical trial in the last 3 months.