Familial Intrahepatic Cholestasis-related Genes Associated with Disease Susceptibility in Hepato-biliary Cancers

Inclusion Criteria: * Instrumental or histological diagnosis of HBCs, defined as primary liver and/or biliary tumors (hepatocellular carcinoma, cholangiocarcinoma, hepatocholangiocarcinoma) occurring in patients without apparent underlying chronic liver disease or in the context of cryptogenic chronic liver disease; * Curative treatment through surgical resection of the neoplasm or liver transplantation * Diagnosis of CCLDs defined as: 1. GGT and/or alkaline phosphatase \>1.5 times the normal values in two or more measurements taken at least 6 months apart, 2. A history of pruritus combined with \[BA\] \>10 mmol/l for a period of ≥6 months. * Obtaining written informed consent Exclusion Criteria: * Other documented causes of chronic liver disease that can justify the clinical phenotype include: Primary biliary cholangitis Primary sclerosing cholangitis IgG4-related cholangiopathy Obstructive jaundice excluded by the demonstration of normal bile duct anatomy Negative virological tests for HBV, HCV, HEV Alcohol abuse Hemochromatosis Wilson's disease Alpha-1 antitrypsin deficiency