Canadian Consortium on Airway Mucus Occlusions in Asthma, COPD and Chronic Cough

Inclusion Criteria: * Males and females (≥18 years old) Asthma: Respiratory physician confirmed diagnosis of moderate-to-severe asthma and Historical objective evidence of asthma and/or specialist/respirologist confirmation (post-bronchodilator reversibility and/or methacholine bronchoprovocation test \<8 mg/mL) COPD: Respiratory physician confirmed diagnosis of COPD that is moderate-to-severe according to GOLD criteria (post-bronchodilator FEV1/FVC\<LLN and FEV1\< 80%pred) and CAT score ≥10 or mMRC score ≥ 2 Chronic cough: Refractory Chronic Cough (RCC) and Unexplained Chronic Cough (UCC) lasting \>1 year with Normal chest radiograph and no airflow obstruction (FEV1/FVC \>Lower Limit of Normal) Healthy participants: No history of respiratory disease or other pulmonary disorders and no use of inhaled medications or corticosteroids with normal spirometry and Modified Medical Research Council (mMRC) Dyspnea Scale =\<1 Exclusion Criteria: * Pregnant or breastfeeding * Current smoker or \>10 yr pack history or smoked within the last 6 months * Exacerbation within 4 weeks of recruitment * Preterm birth (≤36 weeks gestation) or perinatal complications * History of other pulmonary disorders * Specialist/Respirologist suspects primary ciliary dyskinesia (PCD) * Current use of mucolytic medications * Memory, cognitive, or psychiatric limitations that may prevent optimal participation * Treatment with current biologic therapy: anti-IgE mAb within 130 days, anti-IL-4/4R, IL-5/5R, IL-13, or TSLP within 2 months or 5 half-lives whichever is longer prior to visit 1