TCM Daoyin for Anxiety/Depression: Psychological Effects and Biological Mechanisms

Inclusion Criteria: * Primary symptoms of anxiety and depression, with a Hamilton Anxiety Rating Scale (HAMA-14) score between 14 and 29 and a Hamilton Depression Rating Scale (HAMD-17) score between 7 and 14. * No prior use of psychotropic medication, or at least six months since discontinuation. * Aged 18 to 65 years (male or female). * Clear consciousness and normal cognitive function, with the ability to communicate effectively, read, express thoughts, and engage in daily activities independently. * Willing and able to comply with blood and stool sample collection. * Voluntary participation, with signed informed consent provided before enrollment. Exclusion Criteria: * Diagnosed anxiety disorder or depressive disorder based on ICD-10 criteria. * Bipolar disorder, psychotic disorders (e.g., schizophrenia), or organic mental disorders (e.g., Alzheimer's disease). * History of alcohol or substance use disorder within the past year, current psychiatric medication use, or high suicide risk. * Severe or unstable medical conditions, including immune or endocrine disorders or other serious illnesses. * Physical or psychological limitations preventing participation in the TCM Daoyin intervention. * Pregnant or breastfeeding women.