Assessing the Correlation Between Phospholipase C Zeta Measurements and Semen Parameters

Couple inclusion criteria: * Presenting to the IVF clinic for IVF/ICSI treatment or routine semen analysis * Semen sample must have a total sperm count of ≥1 million sperm/ml * Availability of excess, leftover sperm after clinical treatment procedures * Couples must have experienced infertility for at least one year and be undergoing fertility treatment * Undergoing standard clinical treatment protocols (including embryo culture and, when applicable, PGTA) with routinely collected treatment outcome data * Sufficient ovarian reserve and/or meeting the clinic's standard criteria for fertility treatment Couple exclusion criteria: * Known genetic disorders affecting fertility * History of vasectomy or other irreversible sterilization procedures * Current use of medications known to affect sperm parameters (e.g., exogenous androgens, chemotherapeutic agents, or other drugs specifically impacting spermatogenesis) * Recent history of chemotherapy or radiation therapy * Female age \>38 years old * Known chromosomal abnormalities or genetic disorders that directly affect oocyte quality or embryo development * Presence of severe uterine or pelvic pathology (e.g., significant uterine anomalies, advanced endometriosis) * Documented ovarian failure or extremely diminished ovarian reserve as determined by AMH levels (AMH \>1 ng/mL, AFC ≥5) * Use of medications or undergoing treatments that could significantly alter oocyte quality or embryogenesis (outside standard ART protocols).