Serum Ferroptosis Biomarkers (GPX4, MDA) in Alopecia Areata

Inclusion Criteria: * Patients aged between 18 and 65 years. * Formally diagnosed with Alopecia Areata based on clinical and trichoscopic examination. * Healthy volunteers with no personal or family history of alopecia or any systemic inflammatory/autoimmune conditions (for the healthy control group). * Patients who have provided written informed consent before any study-related procedures. Exclusion Criteria: * Use of topical corticosteroids, intralesional steroid injections, or topical calcineurin inhibitors within the last 1 month. * Use of systemic immunosuppressive therapies, systemic corticosteroids, or JAK inhibitors within the last 3 months. * Presence of any other co-existing active autoimmune or inflammatory skin diseases (e.g., Psoriasis, Vitiligo, or active autoimmune thyroiditis with elevated TSH levels). * History of malignancy, active severe infection, chronic hepatic failure, or chronic renal failure. * Pregnancy or lactation. * History of heavy smoking or uncontrolled/regular use of antioxidant or vitamin supplements (e.g., Vitamin E, Vitamin C, CoQ10, NMN, resveratrol).