Microbiome and Enteric Signatures in Sepsis-associated Hepatorenal Injury

Inclusion Criteria: \- For Sepsis Patients: 1. Age ≥ 18 years； 2. Admitted to the Intensive Care Unit (ICU) and meets the Sepsis-3 diagnostic criteria (an acute change in total Sequential Organ Failure Assessment \[SOFA\] score ≥ 2 points consequent to the infection). 3. Diagnosed with sepsis within 24 hours prior to enrollment. 4. Informed consent signed by the patient or a legally authorized representative. For Healthy Volunteers: 1. Age ≥ 18 years. 2. No chronic underlying diseases (including liver, kidney, gastrointestinal, or immune-related disorders). 3. No use of antibiotics or probiotics, and no history of acute infection within 1 month prior to enrollment (to ensure baseline consistency). 4. Informed consent signed by the volunteer. Exclusion Criteria: 1. History of chronic liver disease (e.g., cirrhosis, chronic hepatitis B/C, autoimmune hepatitis, hepatic carcinoma). 2. History of chronic kidney disease (e.g., glomerulonephritis, IgA nephropathy). 3. History of inflammatory bowel disease (including ulcerative colitis and Crohn's disease) or previous major intestinal resection. 4. Patients with malignant tumors currently receiving chemotherapy or radiotherapy. 5. Expected survival time of less than 72 hours. 6. Pregnant or lactating women. 7. Concurrent participation in other interventional clinical trials.