Microbiological and Biochemical Profiling of Amniotic Fluid in Women With Short Cervix or Premature Rupture of Membranes

Inclusion Criteria: * Singleton pregnancy; * Age \> 18 years; * Pregnant women diagnosed with cervical incompetence (via ultrasound criteria) with cervical length \<24 mm, before 24 weeks of gestational age, with intact amniotic membranes and absence of uterine contractions; * Pregnant women diagnosed with p-PROM (via clinical criteria and/or with a qualitative immunoassay vaginal test) before 34 weeks of gestational age; * Healthy pregnant women with uncomplicated obstetric history who underwent amniocentesis to exclude chromosomal abnormalities; * Informed consent to participate in the study. Exclusion Criteria: * Multiple pregnancy; * Patients with contraindications to amniocentesis, including severe bleeding disorders or placenta previa; * Patients with known fetal anomalies incompatible with life; * Patients with sepsis or clinical diagnosis of chorioamnionitis;