A Prospective Diagnostic Trial of Al[¹⁸F]F-NOTA-folate PET in Patients With Suspected Cardiac Sarcoidosis and Infective Endocarditis

Inclusion Criteria: * Ability to provide written informed consent * Suspected cardiac sarcoidosis group: clinical suspicion of cardiac sarcoidosis requiring diagnostic evaluation, including cardiac magnetic resonance and/or \[¹⁸F\]FDG PET * Suspected infective endocarditis group: clinical suspicion of native or prosthetic valve infective endocarditis requiring diagnostic evaluation * Healthy volunteers group: * Age above 50 and post-menopausal (women) * No history of cardiovascular disease, systemic inflammatory disease, malignancy, and no active infection Exclusion Criteria: * Pregnancy or breastfeeding * Known hypersensitivity to folate-based compounds or investigational medical product excipients * Unwillingness or inability to use effective contraception during the study participation period, in participants of childbearing potential * Severe renal impairment (CKD-EPI GFR \<20ml/min) * Clinically unstable condition that, in the opinion of the investigator, would make study imaging unsafe or not feasible (e.g., haemodynamic instability, decompensated heart failure, uncontrolled clinically significant arrhythmia, suspicion of acute coronary syndrome) * Systemic immunosuppressive therapy for more than 7 days prior to baseline Al\[18F\]F-NOTA-folate PET imaging * Inability to complete protocol-defined study procedures * Women of childbearing potential must have a negative pregnancy test prior to tracer administration